I presume it is occurred in vivo, as that was the condition of samples on receipt in the lab. But I don’t see how it could be compatible with life.

 

The repeats were also the same (totally lysed)-but the patient died soon after.

      

 

From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Mohammad Al-Jubouri
Sent: 15 June 2009 16:49
To: [log in to unmask]
Subject: Re: Samples TOTALLY haemolysed

 

Ian,

 

Do you mean that this was an in vitro phenomenon and the patient's Hb, platelets, coagulation, UEs and LFTs were normal prior to this happening?

 

thanks

 

Mohammad

 

 

Thank you both,

 

But in these scenarios are ALL the erythrocytes destroyed (s they were in this case)?

I have only ever seen this occur when we have deliberately destroyed cells, in vitro,  by freezing the sample.

 

Ian

 

From: "Bertholf, Roger" <[log in to unmask]>
To: Mohammad Al-Jubouri <[log in to unmask]>; [log in to unmask]
Sent: Monday, 15 June, 2009 16:13:29
Subject: RE: Samples TOTALLY haemolysed

That was my thought as well. Extravascular gross hemolysis will almost certainly produce potassium concentrations near the technical limits, whereas intravascularly hemolyzyed specimens should have a normal potassium, due to physiological mechanisms that maintain potassium homeostasis. We have seen this several times when we get multiple hemolyzed specimens on a patient.

 

Roger

 

Roger L. Bertholf, PhD

Professor of Pathology

University of Florida Health Science Center/Jacksonville

 

From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Mohammad Al-Jubouri
Sent: Monday, June 15, 2009 11:01 AM
To: [log in to unmask]
Subject: Samples TOTALLY haemolysed

 

Dear Ian,

 

This could be due to haemolytic-uraemic syndrome or microangioapthic haemolytic anaemia associated with  metastatic adenocarcinoma of the prostate.

 

reagards

 

Mohammad

 

 

Dr. M A Al-Jubouri, MB ChB, MSc, FRCP Edin, FRCPath
Consultant Chemical Pathologist

 

Dear all,

 

Any ideas here:-

 

We received a number of blood samples recently for a patient with disseminated Ca prostate that were totally haemolysed. The white cells were intact but the RBCs were all lysed. FBC, Serum and coag samples all showed the same phenomenon.

 

We got repeat bloods and they were the same. The patient died soon after.

 

What is the likely cause of the haemolysis – any ideas??

 

Best wishes

 

Ian Barlow

Scunthorpe

UK


 

 

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------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/