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Hello all, With regard to the issue of my ROI disappearing when I attempted to coregister it as "other image", someone suggested drawing a VOI on multiple slices of the T1 image, which I did in DTIstudio; this worked and I was able to see the ROI after coregistration of the T1 with the b0 image, using the ROI as "other image". However, the ROI is flipped anterior-posterior after this coregistration, despite the fact that the T1 is well registered with the B0. I was assuming that whatever transformation matrix was generated for coregistration of the T1 with the b0 would simply be applied to any "other images" specified, but it doesn't look that way. Does anyone have a suggestion on how I might be able to fix the flipped ROI and complete the analyses? Thanks much - Sarah