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Thank you very much for the answer John. What do you think about this approach that I have developed in my head couple of minutes ago: Assuming that T2 matfile contains the transformation to T1 space, If I do an additional co-registration on it, the co-registration matrix should be very close to identity matrix, right? Now if I define a least-square error criteria, I should be able to detect the errors. Appreciate your thoughts on this -P -----Original Message----- From: John Ashburner [mailto:[log in to unmask]] Sent: Monday, May 18, 2009 1:54 PM To: Mojabi, Pouria; [log in to unmask] Subject: Re: [SPM] transformation matrix > Let's assume you have a T1 and T2 image (image, header and matfile), you > have done som_coreg on T2 and updated its matfile. Now T2's matrix has > the transformation to T1 space which makes it appropriate for > re-slicing. > > My question is how can you Error check that Transformation matrix, > meaning looking at T2 matrix how can you tell if it has been > co-registered to T1 ? You can't from looking at the matrices and headers. To keep the format simple, we don't include that kind of history in the headers. In principle though, you could retain all of the job (batch) files to keep a record of what has been done. This is not yet the default behaviour in SPM, but it is something that I considered implementing. The Check Reg button is probably the most reliable way of telling if images are in register with each other. Best regards, -John