Hi,

>From the experiance of mine I can tell you that the crystal size sometimes matters between these two methods. Hanging drop may yield bigger crystals than sitting drop, that may be due to the evaporation rate(surface area). Hanging drop allow us to set different protocols also like free interface diffusion, area covered by the drp etc.

These informations are gained purely by experiance.

cheers
Deb


On Thu, 30 Apr 2009 20:40:35 +0530 wrote
>I have noticed that a significant majority of crystallizations are done in
>hanging- rather than sitting-drop configuration, and considering the
>significant extra labor involved in hanging drops, can only understand this
>preference as a historical bias. I understand that sometimes one technique
>works and not the other, but all things being equal, why is hanging drop
>still "hanging around?" Any insights appreciated...
>
>Jacob Keller
>
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>Jacob Pearson Keller
>Northwestern University
>Medical Scientist Training Program
>Dallos Laboratory
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