Re: 65% MPD in 0.1M Acetate buffer pH 3.6
These conditions can crystallize salt impurities present in the protein
buffer.
The first step is to ensure that the crystals are protein.
If they are protein the next step is to reduce the MPD concentration and
seed
the drops if no crystals appear. This should allow you to pass from
rosettes to
single crystals.
On Mon, 18 May 2009 12:42:32 +0200, james09 pruza <[log in to unmask]>
wrote:
> Deal all,
>
> Sorry for the non-ccp4 query.
>
> I am new to this field and need some suggestions regarding the
> improvement
> in the crystallization qual
> I have crystallized a protein with 65% MPD in 0.1M Acetate buffer pH 3.6
> at
> 4 degree. The florets appear after 2 days and covers the whole drop. What
> should I do for the optimization. Its a 20kDa protein.
> The needles are clusters and very long.
> Please suggest how what are the various tricks one can apply.
>
> Thanks in advance for the suggestions.
>
> James pruza
--
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