 |
 |
Maybe I'm wrong. Myself solved structure with 1Zn/400aa. In Pete's case 1Zn/570 aa structure was solved at low resolution. I also heard some other cases of 1Zn for > 400 aa. But for Se, I never heard 1se for > 300aa. Se could be partially incorporated or have mixed oxidation state. This could affect phasing, but could be avoided in most cases. Guangyu On 5/14/09 4:48 PM, "Tommi Kajander" <[log in to unmask]> wrote: > ...Based on what? i doubt thats true. > > Quoting "Guangyu Zhu" <[log in to unmask]>: > >> Zn and Se have similar f' and f''. Why Zn seems have much better phasing >> powder in practice? Is it just because Zn always binds tightly on protein >> but Se might have higher b-factor? >> >> Guangyu >> >> -- > Tommi Kajander, Ph.D. > Macromolecular X-ray Crystallography > Research Program in Structural Biology and Biophysics > Institute of Biotechnology > P.O. Box 65 (Street address: Viikinkaari 1, 4th floor) > University of Helsinki > FIN-00014 Helsinki, Finland > Tel. +358-9-191 58903 > Fax +358-9-191 59940 > >