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I have 3 groups: subjects, relatives and controls. We ran separate t- tests to get our results, but now I would like to show them on one image and when I overlay them they are slightly "misregistered". I am assuming this is because the registration is a little different in each comparison. I would also like to extract the values from regions of significant between group differences. Is it valid to run tbss_2- tbss_4 on all subjects (all 3 groups together) and then fslsplit/fslroi the groups that I want, run analysis, generate images and extract values for graphs? Thanks, Naama Naama Barnea-Goraly M.D. Instructor Center for Interdisciplinary Brain Sciences Research Stanford University Division of Child and Adolescent Psychiatry 401 Quarry Rd. MC 5795 Stanford University School of Medicine Stanford, CA 94305-5795 Phone: (650) 736-1874, fax: (650) 724-4794 CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Stanford Medical Center immediately by telephone at (650) 725-5722 and destroy all copies of this communication and any attachments. Thank you.