发件人: CCP4 bulletin board [mailto:[log in to unmask]] 代表 Liew Chong Wai
发送时间: 2009年4月24日 11:47
收件人: [log in to unmask]
主题: [ccp4bb] Cryo-protectant

Hi all,

Good day

I used MPD as a cryoprotectant (20%, 30%) for my crystal. However, there is no diffraction signal at all. Without the MPD cryo, i still manage to get 5angstrom, but it has very strong ice-ring signal. I used glycerol (15%, 20%, 25% and 30%) before, but it cracked the crystal.

Please advice.

Thanks 

Liew