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If you are uncertain, just freeze your buffer :-) Very old trick from the stone age of crystallography. Long time ago ~ 1999 I cryoed one crystal with 1.5 M hexanediol but there was also 10% glycerol around. Jürgen On 2 Apr 2009, at 14:56, HanJie_Heng Chiat Tai wrote: > Hi, Jim, > > What's the concentration? I know that [hexanediol] between 2.5 - 3.4 > M no additional cryoprotectant is required. > > But in my case my hexanediol conc is only 2.1 M > > Rgds, > HengChiat Tai (HanJie) > > > > > > Date: Thu, 2 Apr 2009 13:11:27 -0500 > > From: [log in to unmask] > > To: [log in to unmask] > > CC: [log in to unmask] > > Subject: Re: [ccp4bb] cryoprotectant for 1,6 hexanediol > > > > The cryoprotectant is 1,6 hexanediol. > > > > Jim > > > > On Thu, 2 Apr 2009, HanJie_Heng Chiat Tai wrote: > > > > > > > > > > > Hi, > > > > > > > > > > > > I have a crystal grown in 2.1M 1,6 hexanediol/0.1 M tro-sodium > citrate (pH 6.5). > > > > > > > > > > > > What's the cryoprotectant can be used to flash cool this crystal? > > > > > > > > > > > > Any online protein crystal cryoprotectant database or published > literature available I can check with to determine to type and > concentration of the cryoprotectant used for my crystal. > > > > > > > > > HengChiat Tai > > > > > > > > > > > > _________________________________________________________________ > > > Rediscover Hotmail®: Get e-mail storage that grows with you. > > > http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Storage1_042009 > > Rediscover Hotmail®: Get quick friend updates right in your inbox. > Check it out. - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Biochemistry and Molecular Biology, W8708 615 North Wolfe Street Baltimore, MD 21205 Phone: +1-410-614-4742 Fax: +1-410-955-3655