Dear Li,
the samples should be devoid of aggregates, at least for the low concentration samples. Some aggregation can be tolerated at the higher concentrations. You should in principle measure samples with different concentrations, such as 1, 2, 5,10 mg/mL.
The high concentration measurements will allow you to get a better signal/noise ratio at the higher s values.
Cheers
Isabelle

**********Dr. Isabelle Billas, PhD.
Department of Structural Biology and Genomics
Institute of Genetics and of Molecular and Cellular Biology
IGBMC/ CNRS(UMR 7104) / INSERM (U964)/UDS/Collège de France

CEBGS
1, rue Laurent Fries
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Li Sheng a écrit :

Dear all,

Is there anyone with experience in small angle x-ray scattering? Could
anyone please tell me the requirements for protein sample purity and
concentration?
Thanx in advance.



Sincerely,
Li

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Institute of Biochemistry and Cell Biology
Institutes for Biological Sciences
Chinese Academy of Sciences
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