Dear SPMers, I have two related questions. 1) if I would like to extract the value of a particular voxel of a single subject defined by an MNI-coordinate (V=spm_vol (con_img_file_name); beta = spm_get_data(V,MNI_XYZ); ), would I need to transform the MNI-coordinate first (to native space) or are all beta-imgs/con- imgs already written in MNI-space? If transformation is necessary, could it be done something like this? V=spm_vol(con_img_file_name); n_voxels = size(MNI,2); XYZ = inv(V.mat)*[XYZ_mni; ones(1,n_voxels)]; XYZ = XYZ(1:3,:); beta = spm_get_data(V,XYZ); 2) In order to determine the "native coordinates" of a voxel using MNI- coordinates, could I just do this: V=spm_vol(normalized_img_file_name); n_voxels = size(MNI,2); XYZ = inv(V.mat)*[XYZ_mni; ones(1,n_voxels)]; XYZ = XYZ(1:3,:); Thanks for your help! Best, Tim