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Hi, I'm curious about the limitations of using anisotropic DTI data for either FA/MD maps or tractography. The data were acquired in the axial plane on a 3T scanner using 21 directions and b=0,1000. The voxdims were 0.9735x0.9735x3.0. Total scan time is 7'00". Are there limitations for using this dataset for ROI-based MD/FA studies? TBSS? ProbTrackx? Deterministic tractography? I hope to get the acquisition changed to 32dir 2x2x2 for new studies. Many thanks for any comments. Jenifer