Hello~ all~~ I'm processing huge DTI data by FSL in fedora10(linux 64bit) but, i have a problem in tbss stage 3 (tbss_3_postreg) i used below command $ tbss_3_postreg -S i have the error message like this~~ . ##################################### merging all upsampled FA images into single 4D image Image Exception : #99 :: Out of memory terminate called after throwing an instance of 'RBD_COMMON::BaseException' /usr/local/fsl/bin/tbss_3_postreg: line 164: 12372 Aborted ${FSLDIR}/bin/fslmerge -t ../stats/all_FA `$FSLDIR/bin/imglob *_FA_to_target.*` creating valid mask and mean FA ** ERROR (nifti_image_read): failed to find header file for 'all_FA' ** ERROR: nifti_image_open(all_FA): bad header info Error: failed to open file all_FA Cannot open volume all_FA for reading! ** ERROR (nifti_image_read): failed to find header file for 'all_FA' ** ERROR: nifti_image_open(all_FA): bad header info Error: failed to open file all_FA Cannot open volume all_FA for reading! ** ERROR (nifti_image_read): failed to find header file for 'all_FA' ** ERROR: nifti_image_open(all_FA): bad header info Error: failed to open file all_FA Cannot open volume all_FA for reading! skeletonising mean FA ** ERROR (nifti_image_read): failed to find header file for 'mean_FA' ** ERROR: nifti_image_open(mean_FA): bad header info Error: failed to open file mean_FA ERROR: Could not open image mean_FA Image Exception : #22 :: Failed to read volume mean_FA terminate called after throwing an instance of 'RBD_COMMON::BaseException' /usr/local/fsl/bin/tbss_3_postreg: line 182: 12381 Aborted $FSLDIR/bin/tbss_skeleton -i mean_FA -o mean_FA_skeleton now view mean_FA_skeleton to check whether the default threshold of 0.2 needs changing, when running: tbss_4_prestats <threshold> ################################ i think this is memory error. The data i used Normal : 84 subjects SVD : 47 subjects 84 vs. 47 Is there any limitation about #of subjects in tbss?? plz solve this problem~! thank you ~!!!