Hi, I've uploaded a zip-file rat_segment.zip (4MB) with the reference Number 650137. It contains: original.nii - The brain image before bias correction spm_debias.nii - After spm debias debias_seg.nii - The semgmented file from spm_debias.nii containing my problem in the dentate gyrus result.pdf - A one page spm check-reg output documenting my problem. Thanks amd Cheers. I really appreciate any help or hint. Wolfgang Mark Jenkinson wrote: > Hi, > > Can you please upload your volumetric image to: > http://www.fmrib.ox.ac.uk/cgi-bin/upload.cgi > and send us the reference number? > Hopefully we'll be able to sort it out easily. > > All the best, > Mark > > > On 29 Mar 2009, at 22:37, Wolfgang Weber-Fahr wrote: > >> Hallo, >> >> thanks a lot for the answer. We tried different parameters for the >> FAST bias field correction, but the segmentation - especially the >> seperation between grey matter and white matter - just was not very >> good. When using the SPM bias field correction prior the >> segmentation, the FAST segmentation looked quite promising, only that >> some of the brightest spots (GM) are clasified as CSF.... .I wonder >> about the algorithm which puts some of the brightest voxels into the >> group with the lowest mean value. I could attach or upload a little >> pdf (70kB) showing the problem. >> Thanks for any help. >> >> Wolfgang >> >> Steve Smith schrieb: >>> Hi - I would not generally recommend using bias field correction >>> before running FAST; FAST explicitly models bias field as part of >>> the processing. >>> >>> Does that improve things? >>> >>> Cheers. >>> >>> >>> On 25 Mar 2009, at 17:41, Wolfgang Weber-Fahr wrote: >>> >>>> Hallo, >>>> >>>> I'm rather new to FSL and try to segment T1-weighted rat brains >>>> using FAST. >>>> (3D FISP TE/TR 4/8 ms 0.15*0.12*0.23 mm^3) >>>> >>>> After brain extraction (BET), some polishing and bias correction >>>> (SPM) I used >>>> fast -t 1 -n 3 -g --nopve .... >>>> since the contrast is not really good I also tried "-t 3" >>>> (PD-weighted). >>>> >>>> The results are pretty good considering the low contrast, but in >>>> some parts of >>>> the images - quite often in the hippocampus (dentate gyrus) - where >>>> the image intensity is actually highest, areas of >>>> pixels are strangely misclassified as CSF (lowest intensity). >>>> >>>> Can anybody give me a clue about what is going wrong? >>>> >>>> Thanks, >>>> >>>> Wolfgang >>>> >>>> >>>> >>>> >>>> >>>> >>>> -- >>>> ---------------------------------------------------------- >>>> Wolfgang Weber-Fahr, Dr.rer.nat. >>>> Central Institute of Mental Health >>>> Neuroimaging Department >>>> J5 >>>> 68072 Mannheim >>>> Germany >>>> >>>> email: [log in to unmask] >>>> phone: ++49 621 1703 2961 >>>> >>> >>> >>> --------------------------------------------------------------------------- >>> >>> Stephen M. Smith, Professor of Biomedical Engineering >>> Associate Director, Oxford University FMRIB Centre >>> >>> FMRIB, JR Hospital, Headington, Oxford OX3 9DU, UK >>> +44 (0) 1865 222726 (fax 222717) >>> [log in to unmask] http://www.fmrib.ox.ac.uk/~steve >>> --------------------------------------------------------------------------- >>> >>> >> >> >> -- >> ---------------------------------------------------------- >> Wolfgang Weber-Fahr, Dr.rer.nat. >> Central Institute of Mental Health >> Neuroimaging Department >> J5 >> 68072 Mannheim >> Germany >> >> email: [log in to unmask] >> phone: ++49 621 1703 2961 >> > -- ---------------------------------------------------------- Wolfgang Weber-Fahr, Dr.rer.nat. Central Institute of Mental Health Neuroimaging Department J5 68072 Mannheim Germany email: [log in to unmask] phone: ++49 621 1703 2961