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Bottomley, Matthew wrote: > Dear Cooters, > > Is WinCoot able to handle glycosylated Asn residues? If I load my > current PDB file that contains an Asn with an N-linked NAG modification > the NAG does not appear bonded to the Asn side-chain (whereas in Pymol > this connection is drawn). The Asn and NAG have different residue > numbers - is that the source of the problem? Any good work-arounds? Dear Matthew, Yes, this is a know issue, there is no work-around (other than the gimp/photoshop :). It is due to be fixed, not that that helps you now. But note that the bond drawing algorithm is different to the restraint generation - so even though the bond is not drawn, can still be used in sphere refinement. Paul.