Sorry for the incomplete information. The template for the model has a 70% sequence identity and is a monomer. We only have SEC-MALLS data to suggest that our molecule forms a trimer in solution. It is only based on this evidence that we wanted to try and model the trimer.

On 10/03/2009, Ed Pozharski <[log in to unmask]> wrote:

I assume that the structural homologue does not form a trimer? If it
does, than the obvious solution is to superpose your model on top of
that trimer.

On Tue, 2009-03-10 at 10:39 +0000, amit sharma wrote:
> Dear All,
>
> Apologies for a non-CCP4 question. I have a 13 kDa helical protein
> that forms a trimer in solution. I have successfully modelled the
> monomer, using one of the structural homologues.. However, I now
> wanted to look at the trimeric model. I have tried to dock the two
> monomers using one of the docking programs, but it obviously failed as
> I was giving the same coordinates for the two molecules to be docked.
>
> Could someone please direct me to program(s) or online server(s) that
> could possibly model the trimer.
>
> Many thanks, in advance....
> --
> Amit Sharma
--
Edwin Pozharski, PhD, Assistant Professor
University of Maryland, Baltimore
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