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Dear All, Sorry for the incomplete information. The template for the model has a 70% sequence identity and is a monomer. We only have SEC-MALLS data to suggest that our molecule forms a trimer in solution. It is only based on this evidence that we wanted to try and model the trimer. Many thanks for the replies. Amit On 10/03/2009, Ed Pozharski <[log in to unmask]> wrote: > > I assume that the structural homologue does not form a trimer? If it > does, than the obvious solution is to superpose your model on top of > that trimer. > > On Tue, 2009-03-10 at 10:39 +0000, amit sharma wrote: > > Dear All, > > > > Apologies for a non-CCP4 question. I have a 13 kDa helical protein > > that forms a trimer in solution. I have successfully modelled the > > monomer, using one of the structural homologues.. However, I now > > wanted to look at the trimeric model. I have tried to dock the two > > monomers using one of the docking programs, but it obviously failed as > > I was giving the same coordinates for the two molecules to be docked. > > > > Could someone please direct me to program(s) or online server(s) that > > could possibly model the trimer. > > > > Many thanks, in advance.... > > -- > > Amit Sharma > -- > Edwin Pozharski, PhD, Assistant Professor > University of Maryland, Baltimore > ---------------------------------------------- > When the Way is forgotten duty and justice appear; > Then knowledge and wisdom are born along with hypocrisy. > When harmonious relationships dissolve then respect and devotion arise; > When a nation falls to chaos then loyalty and patriotism are born. > ------------------------------ / Lao Tse / > > > > -- Amit Sharma