Thank you very much for your advice. I checked "$PATH" with echo. It showed "/usr/local/fsl/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games", but when I restart the computer, the path "/usr/local/fsl/bin" is just disappeared./usr/local/fsl/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games Using "nano .bash_profile", it just shows that # FSL Configuration FSLDIR=/usr/local/fsl PATH=${FSLDIR}/bin:${PATH} . ${FSLDIR}/etc/fslconf/fsl.sh export FSLDIR PATH in the middle of the page. I can't find the problem. Besides, I use fsl-4.1.2-centos5_32.tar under Ubuntu8.10. Thank you again for your kindness.