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This would require that the ROIs were in alignment with your T1-w image prior to the coregistration. Another explanation would be that the headers (or mat files - depending on SPM version) or the ROI image were write-protected. Best regards, -John On Friday 16 January 2009 14:48, Richard Binney wrote: > Hi, > > I'm using 'coregister:estimate and reslice' to tranform ROIs in native T1 > space to DWI space. Using check-reg and the automatic graphic, it appears > to have successfully registered the T1 to the DWI b=0 image. I have > several ROIs that I have put as 'other images'. Although it has worked in > the past, the registration of the ROI places the ROIs in a position that > not only doesn't fall in the correct place on the DWI, it no longer fits on > my resliced or my original T1 image. > > I found inconsistencies in the success of this before, but 'try and try > again' let me to successful results. Now I can't get it to work at all. > > Does anyone have any ideas what might be happening? > > I am using nearest neighbour interpolation so that ROIs remain in binary > form. > > Best > > richard