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Couldn't the lines be explained most simply by extreme mosaicity, perhaps severely anisotropic? If not, why not? What were the values obtained in integration? Jacob ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [log in to unmask] ******************************************* ----- Original Message ----- From: Margriet Ovaere To: [log in to unmask] Sent: Wednesday, January 28, 2009 7:51 AM Subject: [ccp4bb] small lines in diffraction pattern Dear all, In the diffraction pattern of crystals of an RNA decamer, small lines appeared (see pictures attached). We've tried different crystals but they all showed the same small lines. �Has anybody seen this�phenomena�before and has got an explanation for it please..? Many thanks Margriet Ovaere ------------------------------------------------------------------------------ ------------------------------------------------------------------------------ Margriet Ovaere Chemistry Department K.U.Leuven Biomolecular Architecture Celestijnenlaan 200 F B-3001 Heverlee (Leuven) Tel: +32(0)16327477 ------------------------------------------------------------------------------ Dear all, In the diffraction pattern of crystals of an RNA decamer, small lines appeared (see pictures attached). We've tried different crystals but they all showed the same small lines. Has anybody seen this phenomena before and has got an explanation for it please..? Many thanks Margriet Ovaere  Margriet Ovaere Chemistry Department K.U.Leuven Biomolecular Architecture Celestijnenlaan 200 F B-3001 Heverlee (Leuven) Tel: +32(0)16327477 Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm