Margriet
This
looks like stacking or shift disorder which can occur when perfect 3 dimensional
order breaks down. For example one can have a situation where the lattice is
preserved in 2 dimensions but the planes can slide with respect to one
another destroying the order in the 3rd dimension, thereby giving rods in
reciprocal space rather than spots. Preservation of order in 1 dimension but
with the 1 D lattices shifted with respect to each other gives diffuse planes in
reciprocal space. If displacements are non random, than the diffuse scatter
might only occur for certain reflections.
As
sharp spots are also present, it is likely that the 3D order is preserved over
much of the sample. To analyse properly one would have to record diffraction
over small angular increments (similar to 3D reciprocal space mapping) and
perhaps identify the hkl value of reflections which exhibit strong, weak or
absent effects of this type.
The
reason for the behaviour can become clear when the structure is known and
weak lattice interactions identified. However, if these effects are preventing
you determining the structure then this statement is not particularly
useful.
Others
will be far better at suggesting means of preventing the effects, which I
suspect is what you would really like to know!
Colin
Dear all,
In the diffraction pattern of crystals of an RNA decamer, small lines
appeared (see pictures attached). We've tried different crystals but they all
showed the same small lines. Has anybody seen
this phenomena before and has got an explanation for it
please..?
Many thanks
Margriet Ovaere
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