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If you will use the Nextal device, I have also modified it something like fig.2 for checking crystals in the drop at room temperature. It is not difficult to make, too. Please see, http://journals.iucr.org/j/issues/2005/02/00/he5324/he5324bdy.html However, getting the film I have used in the note is not easy. The film was SUMILITE FS-1700 film of 50um thickness (Sumitomo Bakelite Co. Ltd). I guess I can not get it in small amounts commercially now. But you can go with any seal tape if you do not care about background, and absorption and permeability of water. Water absorption of the film is 0.01% at 313 K, 90% relative humidity, and water vapour permeability is only 0.4 g/m2/24h. http://www.sumibe.co.jp/english/products/pdf/03_10_1700.pdf I found I did not cite the histric paper in my labo-note... Nobuhisa Watanabe, PhD. === Synchrotron Radiation Research Center Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering Nagoya University C1-3(651) Furo-cho Chikusa-ku, Nagoya 4648603 Japan Email: [log in to unmask] Fax: +81-52-789-5286 On 2009/01/23, at 2:03, James Holton wrote: > Something that is missing from your list is a remarkable device > pictured as figure 2 in: > Perutz, M. (1985) "Early days of protein crystallography." /Methods > in Enzymology/ *114* 3-18 <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=4079771 > >. > > which is actually a reproduction of figure 5 from: > J. D. Bernal and I. Fankuchen (1941) J. Gen. Physiol., 25, 111-146. > > This cell is not difficult to make. I made one using a plastic ring > (to replace the brass) glued to an iron nut (to attach to the > goniometer magnet), a piece of Scotch Tape for the exit window and > the cover slip from the hanging drop setup of interest as the > entrance window. All you do is move the cover slip from the tray to > the ring and then mount it up. The excess vacuum grease from the > tray makes the seal. Maybe put ~10 uL of well solution on the inner > edge of the ring before closing it to keep the humidity stable. The > background (especially from glass) is high but you have the > advantage of never touching the crystal before shooting it. We do > use this device from time to time at the beamline. > > I thought I had invented something new until I saw Perutz's paper. > It was then that I realized that my "invention" was one of the FIRST > mounts ever used to get diffraction from a protein crystal: > > J. D. Bernal and D. Crowfoot (1934) Nature (London) 133, 794. > > > -James Holton > MAD Scientist