hello all,
I would like to thank everyone for all suggestions.
Summary:
1 - MiTeGen Tools - MicroRT™ Room Temperature Mounting System
- crystals slipping ? (suggestion of juan: amongst other things, problem resolved by using micromesh loops instead of using standard loops)
- uses polyester capillary which scatters xrays less than the glass
- works quite well
- everyting is reasonably airtight
- much easier to do than the old way.
links:
http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html
http://mitegen.com/products/micrort/micrort.shtml
2 - coat the crystal with paratone oil and mount the crystal in a standard cryoloop
- no special tools required
- you can use the same crystal to collect under cryo-conditions and directly compare the impact of cooling the crystal
3 - the free mounting system (FMS) from Proteros Biostructures: a humidity control device
- it does not have highly volatile components in the conditions
- room temperature not too difficult
links:
http://www.proteros.com/articles.php?sid=18&lang=de
contact: [log in to unmask]
4 - the humidifier device HC1b
- developed at the EMBL
- available at ESRF
- easy to use
links:
http://www.esrf.eu/UsersAndScience/Experiments/MX/special-setup
technical information: [log in to unmask]
5 - test crystal directly on plate
- japan
http://journals.iucr.org/d/issues/2002/10/01/issconts.html
http://www.labo.co.jp/contents/direct_ex.html
contact: [log in to unmask]
- France, Grenoble
explanations
http://www.natx-ray.com/products/G-Rob_2D.html
the movie
http://www.natx-ray.com/products/G-Rob_2D_movie.html
a prototype, commercialized by Natx-ray, is available on FIP beamline (http://www.esrf.eu/exp_facilities/BM30/).
contact: [log in to unmask]
Best regards,
Cédric
--
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)
tél: +32 (0)2 5273634
fax: +32 (0)2 5267273
