 |
 |
You lose about 1 microlitre of water per well per day with polystyrene Linbro plates - it goes through the plastic Polypropylene and COC are better (or maybe worse if the evaporation encourages crystallization) -- For information and discussion about protein crystallization and automation, please join our bulletin board at http://groups.google.com/group/oryx_group/members_invite?hl=en [log in to unmask] Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 > -----Original Message----- > From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of > Diana Tomchick > Sent: 15 January 2009 21:56 > To: [log in to unmask] > Subject: Re: [ccp4bb] Crystallography plates, hanging drop but > templated sealing film. > > Are you saying that you'd like to re-use the plate with the original > screening solutions, or that you plan to clean the plates, then > dispense fresh screening solutions? > > If you would like to re-use the original screening solutions, > beware...after many weeks, they will not be the same concentrations > (and in some cases, not even the same pH) as they were when first > dispensed into the plates. Slow evaporation of water occurs through > the plastic of the plates as well as through the tape. > > Diana > > > On Jan 15, 2009, at 3:34 PM, Francis E Reyes wrote: > > > Does such a thing exist? A 24-well microplate configuration where in > > substitution of glass cover slips, you have a roll of tape templated > > such that there are circular areas where you can add your protein > > where there is no adhesive, but there is adhesive everywhere else? > > > > This may be a nightmare for plate manufacturers, but to reuse the > > plate, you just throw away the film and tear a new one. > > > > Thanks! > > FR > > > > --------------------------------------------- > > Francis Reyes M.Sc. > > 215 UCB > > University of Colorado at Boulder > > > > gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D > > > > 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D > > * * * * * * * * * * * * * * * * * * * * * * * * * * * * > Diana R. Tomchick > Associate Professor > University of Texas Southwestern Medical Center > Department of Biochemistry > 5323 Harry Hines Blvd. > Rm. ND10.214B > Dallas, TX 75390-8816, U.S.A. > Email: [log in to unmask] > 214-645-6383 (phone) > 214-645-6353 (fax)