Hi,
Thanks for the suggestion. Here is more info: It is a soluble protein, a monomer has 3alpha and 3 beta subunits, and exists in solution as a dimer. The technique is hanging drop, vapor diffusion.  And the  mother liquor is ~ 0.1 mM cacodylate pH 6.2ish, MgCl2 and PEG 20k.

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Hello,

The information you provided to us here is not enough. there are so many 

parameters and 

so many means to optimize 2D crystal. All of which depends on what kind of 

protein you 

are working on (membrane associated or soluble) and what kind of technique that 

you use 

to grow your crystal (lipid monolayer, dialysis or else).



I guess unless you give us more info about your crystal otherwise it will be 

quite difficult 

for us to help you optimize your condition.



Sincerely,

Puey