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You don't have EDTA left over from your protease inhibitor, do you? Some commercial cocktails include it. You may have to read the fine print. James On Jan 27, 2009, at 1:00 PM, Fred wrote: > Hi ccp4 list, > I am trying to purify a his-tag protein by metal affinity > chromatography. The protein was expressed in inclusion bodies and > its his-tag doesn't bind the Qiagen Ni resin in denatured conditions > (urea 8M or GndHCl 6M). Playing with NaCl and detergents didn't help > much. > Any help is appreciated. > Fred