Dear all,<br />I seek your advice regarding the choice of the best&nbsp;connectivity method to address a specific question.<br />We have 45 subjects and 3 ROIs : A, B, and C.<br />We first computed a physiological interaction between A and B and found that C was 'activated' by a negative interaction between A and B.<br />According to the literature, there is no anatomical connection between A and B but there is connection between A and C as well as between B and C.<br />We have therefore put forward two possible interpretations :<br />1) Activity in A decreases functional connectivity between B and C<br />or 2) Activity in&nbsp;B decreases functional connectivity between A and C<br />First : Are we correct ?<br />Second :&nbsp;How can we disentangle these&nbsp;2 interpretations ?<br />Thank you in advance !<br />Best,<br />C&eacute;dric