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Yes!, there is: the fraction of occupied protein with substance can be calculated: S / (S + Km) with S being the concentration of the compound. So, if S = Km, half of the sites are occupied (it follows from Michaelis-Menten theory). In order to saturate the enzyme for 90,90909 % with the compound: 1) S = 10 x Kd (concentration of S at least 10 times the Kd) and 2) S > P (total concentration of S must be larger than total concentration of protein or "binding sites") Depending on the solubility of the compound, this is not always possible. In such a case, you need to use DMSO and/or add solid compound to the protein solution and leave it for quite some time for the compound to finally bind to the protein. - J. - yangliuqing wrote: > Hello,everyone, > I have a question for cocrystallization, is there some relationship > between Km value and substrate concentration when making > cocrystallization? How can I know the substrate is enough for binding? > Thank you very much! > liuqing > > ------------------------------------------------------------------------ > 八卦娱乐包打听，MSN资讯速递帮你忙！ 了解详细！ > <http://im.live.cn/newsexpress> -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451-5004065, Fax: +49-451-5004068 Http://www.biochem.uni-luebeck.de Http://www.iobcr.org Http://www.selfish-brain.org Http://www.opticryst.org -- If you can look into the seeds of time and say which grain will grow and which will not - speak then to me (Macbeth) --