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Thanks Eleanor and everyone else that replied privately. Twinning was a common thought and the data was not by several tests. The MR was solved by getting better data, clean 2.8A vs. the 3.0A I had. With this data phaser was able to solve it easily in R32 with 3 molecules. There was not significant conformational change. I believe the difficulty with the earlier data was due to a translational NCS, but am not completely sure. The density is not great and the R factors very high. I did try refining in R3 and the density and statistics didn't improve. Shane > -----Original Message----- > From: CCP4 bulletin board [mailto:[log in to unmask]] On > Behalf Of Eleanor Dodson > Sent: Monday, November 17, 2008 2:03 AM > To: [log in to unmask] > Subject: Re: [ccp4bb] difficult MR in R32(H32) > > Answer is NO to difficulty; H32 is no easier or harder in > principal than any other SG.. > > However do you have a NCS translation? or twinning? > > Is your model likely to be an oligomer? > Eleanor