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Hi Jason,

- run your model through TLSMD server to identify TLS domains (it will produce PHENIX friendly TLS groups selections);
http://skuld.bmsc.washington.edu/~tlsmd/

- use these selections for TLS refinement in PHENIX:
http://www.phenix-online.org/documentation/refinement.htm
for example, the command is:

phenix.refine model.pdb data.hkl strategy=individual_sites+individual_adp+tls  tls_selections.txt

Pavel.


On 10/17/2008 3:10 AM, [log in to unmask] wrote:
[log in to unmask]" type="cite">
Hi All,
 
I just refined a structure at 2.95A and getting a good R-work and R-free(0.236 and 0.274). When I checked the PDB, I found many  residues have high temperature factor over 100. How can I use Phenix or CNS fix those high temperature factor for further refinment?
 
Thank you
Jason
 

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