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A PhD Position in Structural Biology is available at Structural Biology Brussels, Vrije Universiteit Brussel, Belgium. We are looking for a highly motivated PhD student interested in biochemical and structural studies on tRNA modifying enzymes and enzyme complexes. The Structural Biology Brussels laboratory (for more info: http://www.structuralbiology.be/) is located at the Free University of Brussels and is affiliated with the Flanders Institute for Biotechnology (VIB). Project description: Transfer RNA (tRNA) molecules are pivotal in the translation of the genetic code into protein sequences. In this process, the recognition of the mRNA codon by the correct tRNA does not solely dependent on the primary sequence of the tRNA molecule. Rather, around 100 different posttranscriptional nucleoside modifications have been described, particularly in the anticodon loop, that can modify cognate codon recognition, stabilize the codon- anticodon wobble base pairing and/or affect aminoacylation properties. The current project will deal with the study of the structure/function relationship of tRNA modifying enzymes from both prokaryotic and eukaryotic origin. To study these often multi-subunit complexes and the interaction with their tRNA substrates, an integrated approach of X-ray crystallography, enzyme kinetics and different biophysical methods to study the protein-protein and protein- nucleic acid interactions, will be used. The position is available immediately Candidates should have a recent Masters degree in Biochemistry, Biology, Chemistry or related. Skills in either molecular biology or protein chemistry & purification or protein crystallography would be an asset. Please send applications (including CV, research experience, and at least two names and contact information for references) to: Wim Versées, Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussel - [log in to unmask]