Hi!
I would suggest to try another temperature or seeding. It worked for me before.
Good luck!
Nurit.
"Anyone who has never made a mistake has never tried anything new. Albert Einstein"
Nurit Mirkin Bril
> Date: Wed, 10 Sep 2008 08:02:53 -0400 > From: [log in to unmask] > Subject: Re: [ccp4bb] crystal size > To: [log in to unmask] > > My usual first approach to increasing crystal size is to decrease > precipitant and increase protein concentration, keeping the mixture in > the proper nucleation zone, but providing more protein for crystal > growth. Minimizing dust or particulates by filtration and centrifugation > of reagents and protein may help, by reducing nucleation sites in the > solution. Crystallization at a lower temperature may also result in > fewer, but larger crystals. Or you could try seeding. > > Cheers, > > -- > ------------------------------------------------------------------------ > Roger S. Rowlett > Professor > Colgate University Presidential Scholar > Department of Chemistry > Colgate University > 13 Oak Drive > Hamilton, NY 13346 > > tel: (315)-228-7245 > ofc: (315)-228-7395 > fax: (315)-228-7935 > email: [log in to unmask] > > > Walter Novak wrote: > > Hi Vince, > > > > There is a very nice paper by Monika Budayova-Spano in Acta Cryst 63D > > 2007 339-347. "A methodology and an instrument for the > > temperature-controlled optimization of crystal growth." > > > > Best, > > Wally > > > > On Sep 10, 2008, at 2:23 AM, Vincenzo Carbone wrote: > > > >> Dear all, > >> > >> > >> > >> I was wondering if anyone had some practical advice in regards to > >> increasing the size of a crystal. Currently my enzyme forms these > >> rather nice cubic and very sharp <0.1mm (in 25% peg 5000, 0.1M > >> ammonium sulphate, 0.1M Tris ph 7.0) crystals following optimisation > >> of the pH and precipitant concentrations of the initial screening > >> condition and by playing with various increasing levels of protein > >> concentration 20-60mg/ml. > >> > >> > >> > >> Has anyone had success with additive screens in increasing crystal > >> size and what were they? > >> > >> > >> > >> Any advice would be useful thanks > >> > >> > >> > >> Vince > > > > > > Walter R.P. Novak, Ph.D. > > Postdoctoral Fellow > > Rosenstiel Basic Medical Research Center > > Brandeis University > > 415 South St. MS 029 > > Waltham, MA 02454-9110 > > Phone: (781) 736-4944 > > Fax: (781) 736-2405 > > > > > > > > > > -
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