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Dear all, I was wondering if anyone had some practical advice in regards to increasing the size of a crystal. Currently my enzyme forms these rather nice cubic and very sharp <0.1mm (in 25% peg 5000, 0.1M ammonium sulphate, 0.1M Tris ph 7.0) crystals following optimisation of the pH and precipitant concentrations of the initial screening condition and by playing with various increasing levels of protein concentration 20-60mg/ml. Has anyone had success with additive screens in increasing crystal size and what were they? Any advice would be useful thanks Vince