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Dear Ron -- On 20 Sep 2008, at 15:59, Ron hudson wrote: > it is happily soluble and donot prcipitate at this pH in the same > buffer. Just a quick question. How does your complex look like under DLS? You might still have soluble aggregates, which will precipitate... you can screen for soluble non-aggregates by varying the pH, and check all of this under DLS. HTH Kind regards. -- Leo -- ------------------------------------------------------------ Chavas Leonard, Ph.D. @ home Research Associate Marie Curie Actions Fellow ------------------------------------------------------------ Faculty of Life Sciences The University of Manchester The Michael Smith Building Oxford Road Manchester Lancashire M13 9PT ------------------------------------------------------------ Tel: +44(0)161-275-1586 e-mail: [log in to unmask] http://personalpages.manchester.ac.uk/staff/leonard.chavas/