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Hi Vince, There is a very nice paper by Monika Budayova-Spano in Acta Cryst 63D 2007 339-347. "A methodology and an instrument for the temperature- controlled optimization of crystal growth." Best, Wally On Sep 10, 2008, at 2:23 AM, Vincenzo Carbone wrote: > Dear all, > > I was wondering if anyone had some practical advice in regards to > increasing the size of a crystal. Currently my enzyme forms these > rather nice cubic and very sharp <0.1mm (in 25% peg 5000, 0.1M > ammonium sulphate, 0.1M Tris ph 7.0) crystals following optimisation > of the pH and precipitant concentrations of the initial screening > condition and by playing with various increasing levels of protein > concentration 20-60mg/ml. > > Has anyone had success with additive screens in increasing crystal > size and what were they? > > Any advice would be useful thanks > > Vince Walter R.P. Novak, Ph.D. Postdoctoral Fellow Rosenstiel Basic Medical Research Center Brandeis University 415 South St. MS 029 Waltham, MA 02454-9110 Phone: (781) 736-4944 Fax: (781) 736-2405