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I feel compelled to add: "rather nice cubic <0.1mm" presumably means they're 90x90x90um? At any state-of-the-art synchrotron beamline with a half-way decently-sized beam, that's an absolute stonker, we rarely optimize more if they're that large. phx. Vincenzo Carbone wrote: > > Dear all, > > > > I was wondering if anyone had some practical advice in regards to > increasing the size of a crystal. Currently my enzyme forms these > rather nice cubic and very sharp <0.1mm (in 25% peg 5000, 0.1M > ammonium sulphate, 0.1M Tris ph 7.0) crystals following optimisation > of the pH and precipitant concentrations of the initial screening > condition and by playing with various increasing levels of protein > concentration 20-60mg/ml. > > > > Has anyone had success with additive screens in increasing crystal > size and what were they? > > > > Any advice would be useful thanks > > > > Vince >