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Hi Amit, Dr. Dauter has several excellent papers about solving crystal structures with halide ions. Use this search string in PubMed (without the quotes) to retrieve them: "dauter [AUTH] AND halide". The anomalous signal of iodide can be detected using a home x-ray source but the anomalous signal of bromide generally requires a synchrotron source. Good luck, Eric __________________________ Eric Larson, PhD MSGPP Consortium Department of Biochemistry Box 357742 University of Washington Seattle, WA 98195 On Thu, 25 Sep 2008, amit sharma wrote: > Dear CCP4bbers, > I have a protein molecule(~9.0 kDa) that crystallized in the presence of > 0.15M KBr and HEPES pH 7.0. Since there is no homologuous structure present, > I intend to perform heavy metal derivatization. I read some literature which > suggested that I could carry out quick soak with 0.5M Sodium iodide. I was > wondering if I could soak my crystals with a similar concentration of NaBr > and collect some low resolution data at the in-house source? In addition > should I try to also introduce other heavy metals? it might be worth > mentioning that my protein carries no methionine/cys residues. > I have no prior experience in doing this. So, it would be of great help if > I could be directed towards literature/protocols pertaining to this. Any > advice/suggestions would be greatly appreciated. > > Thanks in advance > -- > Amit Sharma >