Sorry yes it was late and after all I'm getting married on Saturday so a little distracted. ;-). Yes of course Matt's right: eddycorrect then bet (on b0) then dtifit. Cheers -------------------- Stephen M. Smith, Professor of Biomedical Engineering Associate Director, Oxford University FMRIB Centre FMRIB, JR Hospital, Headington, Oxford. OX3 9 DU, UK +44 (0) 1865 222726 (fax 222717) [log in to unmask] http://www.fmrib.ox.ac.uk/~steve ---------------------- On 19 Aug 2008, at 01:24, Matt Glasser <[log in to unmask]> wrote: > I didn't see Steve's reply, but I'm also sure he didn't mean to say > to run > DTIFIT before BET, as a brain mask from BET is one of the inputs for > DTIFIT. > :) > > Peace, > > Matt. > > -----Original Message----- > From: FSL - FMRIB's Software Library [mailto:[log in to unmask]] On > Behalf > Of Steve Smith > Sent: Monday, August 18, 2008 2:51 PM > To: [log in to unmask] > Subject: Re: [FSL] FLIRT co-registration of DWI dataset > > Hi - you should in general align all the different directions and b- > values to each other, and run dtifit, before running BET. > Cheers. > > > On 18 Aug 2008, at 20:04, Megan Herting wrote: > >> Hi. I was trying to prepare my data for dtifit and have seemed to >> run into a >> problem and was looking for some advice. >> >> I have 4 averages consisting of 1 b0 and 20 gradient directions >> (b1000), >> before averaging these together to make one large DWI 4D file, I was >> looking >> to strip each of the 4 b0 images (using BET), align each b0 image to >> the first >> b0 and then apply those parameters to the rest of the gradient >> directions >> (using FLIRT and applyxfm4D). (See script below). >> >> While BET seems to have correctly stripped each b0, after running >> flirt to align >> the b0 and apply it to the rest of the gradient directions, my >> output file >> (${subj}.${DTI}.FG.EC.CR.nii.gz) has large pieces of skull around >> the frontal >> lobe and even the eyesocket/ball, while the superior, posterior, and >> inferior >> brain areas seem to be fully stripped. Any ideas what I am doing >> wrong, or >> how to fix it? >> >> Thanks in advance! >> -Megan >> >> Below is the part of my script I seem to be having trouble with... >> >> >> foreach DTI ( ${runnames} ) >> echo +_+ "BET ${DTI}" >> bet ./${subj}.${DTI}.FG.EC.nii.gz ./ >> ${subj}.${DTI}.FG.EC.STR.nii.gz -m -f 0.3 >> end >> >> #Co-register stripped averaged 1st b0 to stripped 2nd-4th averaged >> b0s >> foreach DTI ( ${DTIs[2]} ${DTIs[3]} ${DTIs[4]} ) >> echo +_+ "Coregister ${DTI} to ${DTIs[1]}" >> flirt -in ${subj}.${DTI}.FG.EC.STR.nii \ >> -ref ./${subj}.${DTI[1]}.FG.EC.STR.nii.gz \ >> -omat ${DTI}.to.${DTIs[1]} >> #Apply b0 co-registration parameters to the rest of the gradient >> directions >> applyxfm4D ./${subj}.${DTI}.FG.EC.nii.gz \ >> ./${subj}.${DTI[1]}.FG.EC.STR.nii.gz \ >> ./${subj}.${DTI}.FG.EC.CR.nii.gz \ >> ${DTI}.to.${DTIs[1]} -singlematrix >> end >> > > > --- > --- > --------------------------------------------------------------------- > Stephen M. Smith, Professor of Biomedical Engineering > Associate Director, Oxford University FMRIB Centre > > FMRIB, JR Hospital, Headington, Oxford OX3 9DU, UK > +44 (0) 1865 222726 (fax 222717) > [log in to unmask] http://www.fmrib.ox.ac.uk/~steve > --- > --- > --------------------------------------------------------------------- >