Defining specific restraints on bonds connecting the chromophore with the rest of the protein worked fine for us in Refmac when we solved a turboGFP structure a couple of years ago. There wasn’t anything special that had to be done – all strictly by the book…

 

Artem

 

 

Hi everybody,

 

we have solved the X-ray structure of a GFP (green fluorescent protein) containing complex. During refinement of the structure we now encountered the problem of connecting the GFP chromophore covalently to the polypeptide chain. We introduced the chromophore to our model as a separate ligand with separate toplogy. So far we could not find a way to integrate it in the primary sequence.

 

Any idea how this problem can be solved? Which programs might be useful?

 

Thank you very much in advance.

 

Best,

 

Sheng & Axel

 

 

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Dr. Sheng CUI     崔胜

 

Department of Chemistry and Biochemistry
Gene Center
University of Munich
Feodor-Lynen-Str. 25
81377 Munich, Germany

 

Tel: +49 89 2180-76984
Fax: +49 89 2180-76999
e-mail: [log in to unmask]

Web:   http://www.lmb.uni-muenchen.de/hopfner/welcome.html

 

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