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How about 90? If it is ok , maybe you need more than 3 or more xtals to collect the whole dataset and merge them together. Otherwise your xtals should be the anisotropic .You'd better to collect images in the better region or screen more xtals to get better ones. Good luck! leo lei feng wrote: > hello everyone, I am seeking help on a crystal decay problem > > I got crystals of a protein , but it decayed very fast in syncrotron > beam. From the initial index, it is P222 space group, I need about 180 > images, its initial diffraction went to about 3 A, and after 70-80 > images, it droped to about 6. I already used low exposure time and it > is in cryo , what I can do ? any suggestion is appreciated. > > BTW. the condition of crystallization is 0.1M NaAC, pH 5.5, 1 M > LiCl, 15% PEG 6000, > > I used 5 seconds / image , which can go to 3 A, if I use 2 seconds, > the initial resolution is only 3.5 A ( beam energy 12kev) > > Thanks > > ------------------------------------------------------------------------ > Got Game? Win Prizes in the Windows Live Hotmail Mobile Summer Games > Trivia Contest Find out how. > <http://www.gowindowslive.com/summergames?ocid=TXT_TAGHM>