Print

Print


Yusuf Akhter wrote:
> Hi Everybody,
> 
> I am refining structure of a protein at 3 Angstrom. I am doing model building in
> Coot.
> After several rounds of refinement using Refmac when I tried to run PROCHECK on
> my partially build model I found that some of the residues are D-amino acids.
> 
> How to change these D-amino acids to L-amino acids??

> Is there any option in Coot for that??

Use Coot 0.4.x or better and the situation would not occur (IIUC).

To fix the problem you have, use Coot 0.4.x or better and use the 
incorrect chiral volume diagnostic and then RSR or regularize the 
affected AA.

Other than that, I would agree with Dale Tronrud.

Paul.