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Hello all, I believe I have a P41 perfectly twinned as a P41 21 2. I'm using the detwin_perfect.inp in CNS in every round of refinement to detwin the original P41 data as I update the single molecule in my asymmetric unit (so far). [1] Should I be able to find the second molecule (from reducing the symmetry from P41 21 2 to P41). Would it be clear as day considering my first molecule is nearly 90% built? Phaser MR gave two solutions in P41 when searching for one molecule in the asu. The LLG is nearly identical. Are these the two twins of my crystal? Should I merge them, detwin, refine and completely rebuild? [2] Should I be using NCS constraints? [3] Would refining with a twin operator on the detwinned P41 data (using phenix.refine) be helpful? Thanks! FR --------------------------------------------- Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D