Lisa Wang wrote: > Hello all, > I have a complex structure with resolution 2.7A The total molecular weight > is 80KD, and about 10% is disordered. I refined this structure to R and > Rfree 25.5% and 29.7% with CNS. I also tried refmac5, but the R and Rfree > have no difference with CNS. I use sfcheck of ccp4 to check this structure. > But sfcheck give the R-factor for all relections 29%. and Rfree 33%. Why > sfcheck and CNS give different R and Rfree? > Which R and Rfree is true? > Best > Lisa > > Re truth - it is all relative! But SFCHECK, CNS and REFMAC all scale the data diferently, and at 2.7A resolution this can make a big difference to the R factors. Eleanor