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Dear Matthew, dear all, I tried ROI analyses with Marsbar using single-subject SPM(2) designs. The preprocessed images have been moved to a different place. I used 'change design path to images' which worked and I got results. However, if I use 'add images to fMRI design', using exactly the same preprocessed images, I get quite different results, although the same design, ROIs and Contrasts have been employed. How is that possible and which results should be used? Thank you in anticipation, Udo