Hi, Be very careful to distinguish between a flip in A-P only vs a flip in A-P *and* L-R. The latter case still retains the same coordinate-system handedness (what we call "radiological" or "neurological") and can be brought into correct alignment using FLIRT, but if it is *only* A-P that is flipped then the result of registration may look OK but actually by L-R flipped, as it cannot undo a single flip, just change which axis the flip is about. I hope this helps. All the best, Mark Vina Goghari wrote: > Hi Mark, > Thanks for the advise. The only thing that is flipped is > Anterior-Posterior. However, it seems that if I allow for a full > search for registration this does not cause a big problem. > > Thanks, > Vina > > Mark Jenkinson wrote: >> Hi, >> >> In Analyze you cannot store orientation information reliably (that's why >> we prefer nifti). FSL has historically adopted the same conventions for >> all Analyze images and these are the same as used in the avg152 images, >> which can be described as "radiological" but that sometimes has >> different >> interpretations by different people. See our FAQ for the precise >> definition. >> >> What I advise is to check the image by looking at it in FSLView, as this >> will show you the labels such as L, R, A, P, S, I. If these are >> correct then >> everything is fine. If the acquisition is not axial then these will >> be wrong >> but check the example_func2standard image and see if that is correct. >> If in doubt, run a test by doing something to the input image, inside >> the brain, >> such as manually editing some voxels to make them very dark which >> you can then use to mark left and right and see if that is propagated >> correctly. >> >> All the best, >> Mark >> >> >> Vina Goghari wrote: >>> Hello, >>> I'm working on a dataset collected a while back, where all the data >>> was stored as Analyze, but none of the orientation information was >>> stored in .hdr file. However, the data was stored in radiological >>> convention (right first then left). I was just wondering how FSL >>> handles this data with out info in the header and whether I have to >>> worry about the data being flipped. In the end the FSL post-stat >>> maps appear to also be in radiological convention. >>> >>> I hope this makes sense! >>> >>> Thanks, >>> Vina >>> >