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Subbu, Take a look at the following for an example of how to proceed. J. Ay et al., Proc. Natl. Acad. Sci. USAVol. 95, pp. 6613–6618, June 1998 Structure and function of the Bacillus hybrid enzyme GluXyn-1: Native-like jellyroll fold preserved after insertion of autonomous globular domain Cheers, Michael **************************************************************** R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 513 Biochemistry Bldg. Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334 Email: [log in to unmask] **************************************************************** On Jul 1, 2008, at 6:29 AM, Narayanan Ramasubbu wrote: > Dear All: > I am working with two enzymes (40 kDa each). The crystal structures > of the two enzymes are known. > I have made a hybrid enzyme of the two individual ones. > This chimeric/hybrid enzyme is soluble and exhibits activity of the > two individual enzymes. > My questions are as follows: > 1. Could I use one of the enzyme's structure to solve the crystal > structure of the hybrid by MR? > 2. Has anyone working with such hybrids (?) noticed any increase in > specific activity of either of the enzymes? > Interestingly, the two enzymes work on the same substrate polymer. > One is a hydrolase another is an esterase. > > Thanks a lot. > Subbu >