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ESI-MS at a precision of +-2 Da should work alone. Best wishes Kornelius On Tue, 1 Jul 2008 19:32:56 +0100 Matthew Chu <[log in to unmask]> wrote: > N-terminal sequencing / MS for intact mass analysis are the only ways that I > can think of..... > > Matt > > 2008/7/1 Klaus Futterer <[log in to unmask]>: > > > We have a 150 kDa protein that reproducibly crystallises at one of the > > Hampton Screen conditions. However, we know from SDS gel analysis that the > > crystals contain only a 45 kDa fragment, that forms through proteolytic > > cleavage over time. We would like to determine the sequence boundaries of > > the fragment. > > > > We believe a combination of N-terminal sequencing plus MS analysis might > > give us the information we need, but I was wondering whether the ccp4bb > > community has other suggestions. > > > > Klaus > > > > > > > > > > > > > > --------------------------------------------------------------------- > > > > Klaus Fütterer, Ph.D. > > > > School of Biosciences P: +44-(0)-121-414 5895 > > University of Birmingham F: +44-(0)-121-414 5925 > > Edgbaston E: [log in to unmask] > > Birmingham, B15 2TT, UK W: www.biochemistry.bham.ac.uk/klaus/ > > --------------------------------------------------------------------- > > > > > > > ---------------------------------------------------------------------------- > Matthew LH Chu > PhD Student > School of Pharmacy and Pharmaceutical Sciences > University of Manchester > ---------------------------------------------------------------------------- ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [log in to unmask] Tel -49 7071 601 323 Fax -49 7071 601 349