Hi Michele!

-did you go in steps from 10 to 40 C? did you monitor drifts while doing that? has the energy range, where you do not observe problems, changed?

In fact I was not planning to do that cause 40ºC seems to be the mono’s basal temperature. In addition, I operated near 15ºC in the past and didn’t notice any appreciable change after that… But I agree a more careful choice will be necessary in the near future!

-I am a bit worried about keeping the crystal at 40 C... did you speak to the engineer who designed the mono about the optimal cooling temperature?

Yes, but no kind of recommendation was informed in this way…

-which is actually your energy range now?

MX2 was projected to operate from 5 to 15 keV. (http://rcsb-biosync-beta.rutgers.edu/lnls/W01B-MX2.html)

-do you have a compton scattering guard on the second crystal? We had one, and it was surprising so see the marks that I had after a year of being installed.

Ooh, unfortunately I don’t have one. I fear for that.

-Check the water flow rate. Too high flow would cause turbulence, and a too low, would not cool your crystal.

We are operating with about 0.8 liters/min. The mono was projected to work with 1L/min.

do you have access to the machine data? electron beam position and so on? this can help to find time correlation.

Yes I have. I’m starting to think what kind of correlation would be elucidative…

Michele, thank you one more for your help. I’ll be pleased to inform you about our progress.

Regards,

Lucas.