Hi Michele!
-did you go in steps from 10
to 40 C? did you monitor drifts while doing that? has the energy range, where you do not observe problems,
changed?
In fact
I was not planning to do that cause 40ēC seems to be
the mono’s basal temperature. In addition, I
operated near 15ēC in the past and didn’t notice any appreciable change
after that… But I agree a more careful choice will be necessary in the
near future!
-I am a bit worried about
keeping the crystal at 40 C... did you speak to the engineer who
designed the mono about the optimal cooling temperature?
Yes, but
no kind of recommendation was informed in this way…
-which is actually your energy
range now?
MX2 was projected to operate from 5 to 15 keV. (http://rcsb-biosync-beta.rutgers.edu/lnls/W01B-MX2.html)
-do you have a
Ooh, unfortunately I don’t
have one. I fear for that.
-Check the water flow rate. Too high flow would cause
turbulence, and a too low, would not cool your crystal.
We are operating with about
0.8 liters/min. The mono was projected to work with 1L/min.
do you have access to the machine
data? electron beam position and so on? this can help to find time correlation.
Yes I have. I’m starting to think what
kind of correlation would be elucidative…
Michele,
thank you one more for your help. I’ll be pleased to inform you about our
progress.
Regards,
Lucas.