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Hmmm... we had a demo of the Malvern Zetasizer instrument here, and to be honest: It did not convince us at all (it is obviously built for non-biological particle analysis).... The Viscotek is also a plug&play device, every pc with a USB is suitable. ..in the end... Viscotek and Malvern are the same company now(!) I guess the price for a DLS instrument is more or less independent of the company.... aren't they all approx. 30-35k euros for a single-cuvette system? Gregor -----Ursprüngliche Nachricht----- Von: CCP4 bulletin board [mailto:[log in to unmask]] Im Auftrag von Roger Rowlett Gesendet: Dienstag, 8. Juli 2008 17:09 An: [log in to unmask] Betreff: Re: [ccp4bb] DLS options? I'll second the recommendation for the Malvern Zetasizer. They are rock-simple and interface with a computer through USB which makes future computer upgrades relatively simple. The are however, not cheap--oops, inexpensive. Cheers, -- ------------------------------------------------------------------------ Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [log in to unmask] Andreas Förster wrote: > Hey Thomas, > > also consider Malvern instruments. Their Zetasizers are really sweet > and work with volumes smaller than 15ul if you use the smallest cuvette. > http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm > > The last DynaPro that I've used, half as old as the universe but > equipped with a 12ul cuvette, gave me really nice results also. Key is, > as you discovered, to keep the cuvette meticulously clean. I used > Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml > beaker, heat to 70C in the microwave with the cuvette inside, let sit > for a while, rinse with water and EtOH, and dry. Only touch with gloves > afterwards. > > The protein sample must be spun down before the experiment. Half an > hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to > avoid bubbles when adding the sample to the cuvette. > > Ah, nice data! > > Hope that helps. > > > Andreas > > > Thomas Edwards wrote: > >> Dear BB, >> >> >> >> Sorry for the off topic question: >> >> >> >> I would like to buy a Dynamic Light Scattering system. >> >> Could people suggest which they like the best and/or which is best value? >> >> >> >> I have in the past used a Protein Solutions Dyna Pro with micro cuvette >> (I would like a micro cuvette option). However, it was very sensitive to >> dust and bubbles, and the cuvette collects dust… >> >> I’ve never tried the one from Precision Detectors. >> >> Any other options? >> >> >> >> Thanks >> >> Ed >> >> >> >> ______________ >> T.Edwards Ph.D. >> Garstang 8.53d >> Astbury Centre for Structural Molecular Biology >> University of Leeds, Leeds, LS2 9JT >> Telephone: 0113 343 3031 >> <http://www.bmb.leeds.ac.uk/staff/tae/>http://www.bmb.leeds.ac.uk/staff/tae/ >> <http://www.bmb.leeds.ac.uk/staff/tae/Research> >> "The doubter is a true man of science; he doubts only himself and his >> interpretations, but he believes in science". ~Claude Bernard >> >> >> >>